| چکیده انگلیسی مقاله |
Myelodysplastic syndromes (MDSs) are a clonal bone marrow (BM) disease characterized by ineffective hematopoiesis, dysplastic maturation and progression to acute myeloid leukemia (AML). Methylation silencing of HRK has been found in several human malignancies. In this study, we explored the association of HRK methylation status with its expression, clinical parameters and MDS subtypes in MDS patients. To study the methylation status of HRK gene, we applied Methylation Sensitive-High Resolution Melting Curve Analysis (MS-HRM) in MDS patients, as well as healthy controls and EpiTect®PCR Control DNA. Real time RT-PCR was used for gene expression analysis. Methylation frequency in promoter region of HRK in patient samples was 20.37%. Methylation of HRK was significantly related to transcriptional downregulation (P=0.023). The difference in frequency of hypermethylated HRK gene was significant between good (10%) and poor (71.42%) cytogenetic risk groups (P= 0.001), advanced stage MDS patients (66.66%) in comparison with early stage MDS patients (2.56%) (P= 0.00), higher- risk MDS group (61.53%) and lower- risk MDS group (7.31%) (P= 0.00). HRK hypermethylation was associated with advanced- stage MDS and downregulation of HRK gene may play a role in the progression of MDS. |
| نویسندگان مقاله |
فرهاد ذاکر | farhad zaker
cellular and molecular research center, iran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
ناصر امیری زاده | naser amirizadeh
blood transfusion research center, high institute for education and research in transfusion medicine, tehran, iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
ناهید نصیری | nahid nasiri
department of hematology, school of allied medicine, iran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
سید محسن رضوی | seyed mohsen razavi
hematology and oncology department, firoozgar hospital, iran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
لادن تیموری تولابی | ladan teimoori toolabi
molecular medicine department, biotechnology research center, pasteur institute of iran, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
مرجان یغمایی | marjan yaghmaie
hematology, oncology and stem cell transplantation research center, tehran university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
رویا مهرآسا | roya mehrasa
blood transfusion research center, high institute for education and research in transfusion medicine, tehran, iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
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