| چکیده انگلیسی مقاله |
Bisphenol-A (BPA) is an industrial xenoestrogen used widely in our living environment. Recently, several studies suggested that BPA has destructive effects on DNA and chromosomes in normal body cells via estrogen receptors (ER). Therefore, BPA could be considered as an important mediator in many diseases such as cancer. However, there are still many controversial issues which need clarification. In this study, we investigated the BPA-induced chromosomal damages in MCF-7 cell line, ER-positive and negative amniocyte cells. Cytotoxicity and genotoxicity effects of BPA were also compared between these three cell groups. Expression of estrogen receptors was determined using immunocytochemistry technique. The cell cytotoxicity of BPA was measured by MTT assay. Classic cytogenetic technique was carried out for the investigation of chromosome damage. BPA, in addition to cytotoxicity, had remarkable genotoxicity at concentrations close to the traceable levels in tissues or biological fluids. Although some differences were observed in the amount of damages between ER-positive and negative fetal cells, interestingly, these differences were not significant. The present study showed that BPA could lead to chromosomal aberrations in both ER-dependent and independent pathways at some concentrations or in cell types yet not reported. Also, BPA could probably be considered as a facilitator for some predisposed cells to be cancerous by raising the chromosome instability levels. Finally, estrogen receptor seems to have a different role in cytotoxicity and genotoxicity effects. |
| نویسندگان مقاله |
سید محسن آقاجانپور میر | seyed mohsen aghajanpour mir
cellular amp; molecular biology research center, health institute, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
ابراهیم ذبیحی | ebrahim zabihi
cellular amp; molecular biology research center, health institute, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
الهه کیهانی | elahe keyhani
genetics research center, university of social welfare and rehabilitation sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم بهزیستی و توانبخشی (University of social welfare and rehabilitation sciences)
هاله اخوان نیاکی | haleh akhavan niaki
cellular amp; molecular biology research center, health institute, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
ایمان باقری زاده | iman bagherizadeh
department of medical genetics and sarem cell research center scrc , sarem women’s hospital, tehran, iran.
سجاد بیگلری | sajjad biglari
genetics research center, university of social welfare and rehabilitation sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم بهزیستی و توانبخشی (University of social welfare and rehabilitation sciences)
فرخنده بهجتی | farkhondeh behjati
genetics research center, university of social welfare and rehabilitation sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم بهزیستی و توانبخشی (University of social welfare and rehabilitation sciences)
سازمان های دیگر: Department of Medical Genetics and Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
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