International Journal of Molecular and Cellular Medicine، جلد ۴، شماره ۴، صفحات ۲۳۵-۲۳۹
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عنوان انگلیسی Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran
چکیده انگلیسی مقاله Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tabriz Pediatric Hospital. The amplification of small subunit ribosomal RNA gene was performed using a nested polymerase chain reaction protocol and its products were digested using two restriction enzymes for Cryptosporidium species and genotype differentiation. Cryptosporidium oocysts were found in 2 (1.76%) children with diarrhea and restriction pattern revealed the presence of C.parvum bovine genotype in both positive fecal samples. The findings indicate that Cryptosporidium parvum is responsible for cryptosporidiosis in children in the study region and probably zoonotic transmission is the predominant route of parasite transmission.
کلیدواژه‌های انگلیسی مقاله Child, Cryptosporidium, genotype, PCR, Iran
نویسندگان مقاله بهروز مهدوی پور | behroz mahdavi poor
department of laboratory science, faculty of paramedicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
سازمان های دیگر: Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University,Tehran | Iran.

محمد اصغرزاده | mohammad asgharzadeh
biotechnology research center and faculty of paramedicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

اسماعیل فلاح | esmaeel fallah
department of parasitology, faculty of medicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

کریم حاتم نهاوندی | kareem hatam nahavandi
department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

جلیل راشدی | jalil rashedi
faculty of paramedicine, tabriz university of medical sciences, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

عبدالحسین دلیمی | abdolhossein dalimi
department of medical parasitology, school of medical sciences, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://www.ijmcmed.org/browse.php?a_code=A-10-532-1&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
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