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International Journal of Molecular and Cellular Medicine، جلد ۴، شماره ۴، صفحات ۲۴۰-۲۴۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Clonal Diversity in Multi Drug Resistant (MDR) Enterococci Isolated from Fecal Normal Flora |
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| چکیده انگلیسی مقاله |
Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based –PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtained from 180 fecal samples. The isolates were identified by biochemical reaction and specific identification was confirmed by PCR with species specific primers. All isolates were subjected to Rep typing and antimicrobial susceptibility tests. Rep-PCR analysis of 180 isolates revealed 93 REP types with forty-five single types (ST1 to ST45) and forty-eight common types (CT1 to 48). Antibiotic susceptibility tests exhibited that 53 (29.4%), 43 (23.8%), 11 (6.1%) and 9 (5%) were resistant to erythromycin, tetracycline, gentamicin and ciprofloxacin respectively but among the isolates, sixteen were multi drug resistant (MDR). These MDR isolates showed 11 Rep types with seven single types and four common types. In addition, 81.2% of MDR isolates were from male subjects and the average age of these persons was more than fifty years. This study showed that 56.2% of MDR isolates were homogeneous with 95 % similarity, and high rate of resistance to tetracycline and erythromycin (81.2%) were observed in these isolates. The concern about these normal flora isolates are the pathogenic potential of these bacteria through the horizontal transfer of antibiotic resistance and virulence genes. |
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| کلیدواژه‌های انگلیسی مقاله |
Enterococcus, Rep-PCR, antibiotic profile, normal flora |
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| نویسندگان مقاله |
میثم حسن نژاد بی بالان | meysam hasannejad bibalan
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
مرتضی اسحاقی | morteza eshaghi
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
جواد صادقی | javad sadeghi
department of microbiology, school of medicine, tehran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهلا اسدیان | mahla asadian
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
تهمینه نریمانی | tahmineh narimani
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
ملیحه طالبی | malihe talebi
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-503-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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