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International Journal of Molecular and Cellular Medicine، جلد ۳، شماره ۳، صفحات ۱۴۶-۱۵۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells |
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| چکیده انگلیسی مقاله |
The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of a recombinant CHO cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin (plant and casein). The amino acid content and the average molecular weight of the peptones chosen were available. In optimized feeding strategies with single peptones, increase 100 % volumetric productivity and 40 % in cell number were achieved. In feeding strategies with two peptones, several combinations stimulated protein productivity more than either peptone alone, depending on the peptone concentration and time of feeding. Some peptones, which did not stimulate productivity when added alone proved to be effective when used in combination. The combined peptones feeding strategies were more effective with peptones of different origin. Our data support the notion that the origin of peptones provides some guidance in identifying the most effective feeding strategies for recombinant CHO cells. |
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| کلیدواژههای انگلیسی مقاله |
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| نویسندگان مقاله |
فاطمه دوامی | fatemeh davami
laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.
لوسیا baldi | lucia baldi
laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.
yashas rajendra | yashas rajendra
laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.
florian m wurm | florian m wurm
laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-252-1&slc_lang=en&sid=en |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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