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International Journal of Molecular and Cellular Medicine، جلد ۳، شماره ۳، صفحات ۱۶۶-۱۷۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties |
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| چکیده انگلیسی مقاله |
Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from serogroup B N. meningitidis was cloned into prokaryotic expression vector pBAD-gIIIA. Recombinant protein was expressed with arabinose and affinity purified by Ni-NTA agarose, SDS-PAGE and western blotting were performed for protein determination and verification. BALB/c mice were immunized subcutaneously with purified rPorA together with alum adjuvant. Serum antibody responses to serogroups B N.meningitidis were determined by ELISA. Serum IgG response significantly increased in the group immunized with rPorA together with alum adjuvant in comparison with control groups. These results suggest that rPorA can be a potential vaccine candidate for serogroup B N.meningitidis. |
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| کلیدواژه‌های انگلیسی مقاله |
Neisseria meningitides , PorA ,Vaccine |
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| نویسندگان مقاله |
آوا بهروزی | ava behrouzi
islamic azad university, science and research branch,tehran, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
سعید بوذری | saeid bouzari
department of biology ,pasteur institute of iran, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
سید داور سیادت | seyed davar siadat
department of microbilogy,pasteur institute of iran, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
شیوا ایرانی | shiva irani
islamic azad university, science and research branch,tehran, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-220-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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