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International Journal of Molecular and Cellular Medicine، جلد ۳، شماره ۱، صفحات ۱-۱۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Mesenchymal Stem Cells as a Feeder Layer Can Prevent Apoptosis of Expanded Hematopoietic Stem Cells Derived from Cord Blood |
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| چکیده انگلیسی مقاله |
Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34+ cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. Apoptosis and cell cycle distribution of expanded cells were analyzed in MSCs co-culture and cytokine conditions and results were compared. Three culture conditions of cord blood HSCs were prepared ex-vivo for 14 days: cytokines (SCF, TPO and Flt3L) with MSCs feeder layer, cytokines without MSCs feeder layer and co-culture with MSCs without cytokines. Expansion was followed by measuring the total nucleated cells (TNCs), CD34+ cells and colonyforming unit (CFU) output. Flow cytometry analysis of stained cells by annexin V and propidium iodide was performed for detection of apoptosis rate and cell cycle distribution in expanded cells. Maximum cord blood CD34+ cells expansion was observed in day 10. The mean fold change of TNCs and CD34+ cells at day 10 in the co-culture system with cytokines was significantly higher than the cytokine culture without MSCs feeder layer and co-culture system without cytokines (n=6, p=0.023). The highest apoptosis rate and the least number of cells in Go/G1 phase were observed in cytokine culture without feeder layer (p=0.041). The expansion of cord blood HSCs on MSCs as a feeder layer resulted in higher proliferation and reduction in apoptosis rate. |
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| نویسندگان مقاله |
رویا مهرآسا | roya mehrasa
university of guilan, rasht, iran.
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
حمیدرضا وزیری | hamidreza vaziri
university of guilan, rasht, iran.
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
آرزو اودی | arezoo oodi
blood transfusion research center, high institute for research and education in transfusion medicine,tehran iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
مونا خورشیدفر | mona khorshidfar
blood transfusion research center, high institute for research and education in transfusion medicine,tehran iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
مهین نیکوگفتار | mahin nikogoftar
blood transfusion research center, high institute for research and education in transfusion medicine,tehran iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
منیره گلپور | monireh golpour
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
ناصر امیری زاده | naser amirizadeh
blood transfusion research center, high institute for research and education in transfusion medicine,tehran iran.
سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-134-1&slc_lang=en&sid=en |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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