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International Journal of Molecular and Cellular Medicine، جلد ۳، شماره ۱، صفحات ۵۱-۵۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Molecular Identification of Macroscopic And Microscopic Cysts of Sarcocystis in Sheep in North Khorasan Province, Iran |
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| چکیده انگلیسی مقاله |
Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformision. The purpose of the study was to determine the molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, we assessed the macroscopic and microscopic cysts of Sarcocystis in sheep carcasses. The digestion method was used for observing of bradyzoite in heart, liver, diaphragm and muscle samples. PCR Analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was done for ten PCR products. Genotypes were identified by Blast search and homology analysis. Macrocyst was seen in two muscle tissues. Digested method and PCR analysis were positive in all samples (heart, liver, diaphragm muscle). Genotyping of ten tissues samples proved that the genotype of macroscopic and microscopic cysts belonged to Sarcocystis gigantea and Sarcocystis tenella, respectively. Microscopic cysts are more prevalent than macroscopic cysts and they can cause enormous economic losses. |
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| نویسندگان مقاله |
پژمان بهاری | pejman bahari
vector-borne diseases research center, north khorasan university of medical amp; laboratory of north khorasan veterinary head office,bojnurd, iran.
میترا صالحی | mitra salehi
vector-borne diseases research center, north khorasan university of medical, bojnurd, iran.
محسن seyyedabadi | mohsen seyyedabadi
vector-borne diseases research center, north khorasan university of medical amp; laboratory of north khorasan veterinary head office,bojnurd, iran.
احمد محمدی | ahmad mohammadi
vector-borne diseases research center, north khorasan university of medical amp; laboratory of north khorasan veterinary head office,bojnurd, iran.
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-151-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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