|
|
International Journal of Molecular and Cellular Medicine، جلد ۱، شماره ۴، صفحات ۲۰۹-۲۱۶
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran |
|
| چکیده انگلیسی مقاله |
Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensitivity pattern to ceftizoxime, ceptazidime. cephotaxim, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibiotics in P. aeruginosa strains isolated from Intensive Care Units (ICU), Shahid Beheshti Hospital, North of Iran. This cross-sectional study was performed from 2011 to 2012. Totally, fifty four P. aeruginosa strains were isolated from ICU at Shahid-Beheshti hospital, Babol, North of Iran. The bacteria were diagnosed based on mobility, pigment production, growth in 420 C, oxidase and catalase tests. PCR analysis was carried out to detect integron genes using hep 35 and hep 36 primers. Also, disk diffusion method was performed to evaluate antibiotic susceptibility of the bacteria using ceftizoxime, ceftazidime, cephotaxime, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibacterial reagents. This study revealed that 20 (37%) P. aeruginosa isolates had integron genes. The antibiotic susceptibility test showed that 52 (96.3%) of the isolates were multidrug-resistant. 12 out of 54 isolated bacteria were resistant to all antibiotics tested. All bacteria were resistant to cefepime (100%) and the highest resistance rate was seen to ceftazidime 92.6% fallowed by cefazolin 96.3%. The lowest resistance rate was observed to ciprofloxacin 38.9%, ofloxacin 44.4%, amikacin 46.3% and ticarcillin 48.1%. According to this study, P. aeruginosa isolates showed high level of antibiotic resistance and the presence of integrons in these strains can explain the influence of these genes in resistance creation. There was a significant association between resistance to cefotaxime, amikacin, ofloxacin, imipenem, ticarcillin, gentamicin and the presence of integrons. |
|
| کلیدواژه‌های انگلیسی مقاله |
Pseudomonas aeruginosa, integrons, drug resistance |
|
| نویسندگان مقاله |
فاطمه مرادیان | fatemeh moradian
department of microbiology science and research branch islamic azad university, guilan, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
الهه فردوسی شاهاندشتی | elaheh ferdosi shahandashti
department of microbiology and immunology, babol university of medical sciences, babol iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
زهرا مولانا | zahra moulana
infectious diseases and tropical medicine research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
معصومه مرادیان کوچکسرایی | massomeh moradian kouchaksaraei
department of microbiology science and research branch islamic azad university, fars, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
فریبا اصغرپور | fariba asgharpour
faculty of para-medicine; babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
علی مجتهدی | ali mojtahedi
department of microbiology, faculty of medicine, guilan university campus, rasht, iran.
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)
رمضان رجب نیا | ramazan rajabnia
infectious diseases and tropical medicine research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
|
|
| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-86-1&slc_lang=en&sid=en |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
1 |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|