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International Journal of Molecular and Cellular Medicine، جلد ۱، شماره ۱، صفحات ۲۱-۲۹
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Anti-influenza Activity of a Novel Polyoxometalate Derivative (POM-4960) |
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| چکیده انگلیسی مقاله |
There are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with FluV (influenza virus) viral binding or its penetration into cell membrane. A series of polyoxometalates compounds such as POM-523 and PM-504 have been synthesized and have showed inhibitory effects on viruses. In this study we examined anti influenza activity of a novel polyoxometalate derivative (POM-4960) synthesized in the Faculty of Chemistry of Damghan University of Basic Sciences.To evaluate the anti-influenza activity of POM, following the treatment of FluV with POM at different temperatures and incubation periods, viral titer reduction was assessed by haemaglutination assay (HA). The 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine TCID50 (tissue culture infective dose) of virus, CC50 (median cytotoxic concentration) of POM, protection percentage and antiviral activity of POM in cell culture. RT-PCR and direct Immunofluorescent assays were performed to evaluate the effect of POM on viral infection and viral RNA load, respectively. POM reduced HA titer near to zero in all cell culture specimens and showed high protection against viral infection of the cells. Reduction in viral infection was confirmed by RT-PCR and Immunofluorescent staining methods. Moreover, this POM derivative has a dual (cumulative) effect on attachment and penetration inhibition compared to other POM’s with just one inhibitory effect. POM-4960 could be considered as a powerful anti-influenza agent with low toxicity and high antiviral potency. |
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| کلیدواژه‌های انگلیسی مقاله |
POM, influenza virus, RT-PCR, immunofluorescent, MTT |
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| نویسندگان مقاله |
سید مسعود حسینی | seyed masoud hosseini
department of microbiology, faculty of biological science, shahid beheshti university, evin, tehran, iran.
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
الهام امینی | elham amini
influenza unit, pasteur institute of iran, 12 farvardin st. tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
معصومه توسطی خیری | masoumeh tavassoti kheiri
influenza unit, pasteur institute of iran, 12 farvardin st. tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
پروانه مهربد | parvaneh mehrbod
influenza unit, pasteur institute of iran, 12 farvardin st. tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
مهسا شهیدی | mahsa shahidi
influenza unit, pasteur institute of iran, 12 farvardin st. tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
ابراهیم ذبیحی | ebrahim zabihi
cellular and molecular biology research center cmbrc , babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-60-1&slc_lang=en&sid=en |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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