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International Journal of Molecular and Cellular Medicine، جلد ۱، شماره ۱، صفحات ۳۰-۳۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Stress response induced by carbon nanoparticles in Paracentrotus lividus |
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| چکیده انگلیسی مقاله |
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now is totally obscure their toxic effect on development. Embryos treated with carbon nanoparticles, RNA preparation, retro-transcription and quantitative real-time PCR. In response to carbon nano-particles exposure, the embryos collected 24 h later showed a 3,07-fold at 5x1012 p and a 1,58-fold at 2.5x1013 p and a 1,92-fold at 2.5x1014 p increase in Pl14-3-3ε transcript levels compared with controls. The Pl14-3-3ε mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilon isoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3ε. Pl14-3-3ε mRNA levels were measured by RT-PCR during development and found to increase from the mesenchyme blastula to the prism stage. Our results confirm the involvement of 14-3-3ε in the stress response elicited by carbon nano-particles. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
elisabetta carata | elisabetta carata
department of biological and environmental sciences and technologies, university of salento, lecce, italy.
bernardetta آنا tenuzzo | bernardetta anna tenuzzo
department of biological and environmental sciences and technologies, university of salento, lecce, italy.
arnò federica | arnò federica
department of biological and environmental sciences and technologies, university of salento, lecce, italy.
alessandro buccolieri | alessandro buccolieri
department of materials science, university of salento, lecce, italy.
آنتونیو serra | antonio serra
department of materials science, university of salento, lecce, italy.
دانیلا manno | daniela manno
department of materials science, university of salento, lecce, italy.
luciana دینی | luciana dini
department of biological and environmental sciences and technologies, university of salento, 73100 lecce, italy.
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| نشانی اینترنتی |
http://www.ijmcmed.org/browse.php?a_code=A-10-31-1&slc_lang=en&sid=en |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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