International Journal of Molecular and Cellular Medicine، جلد ۹، شماره ۱، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Computational Approach for Rational Design of Fusion Uricase with PAS Sequences
چکیده انگلیسی مقاله Tumor lysis syndrome is a life-threatening condition for humans due to the lack of urate oxidase. In this study, several variants of PASylateduricasefrom the Aspergillus flavus species were analyzed computationally to find the appropriate fusions to solve short half-life and stability concerns. The Ab initio method was performed using Rosetta software to structurally characterize the PAS sequences. The 3D structures of fusions were predicted for fused C- or N-terminally PAS sequences in different lengths to the uricase. The refinement and energy minimization steps revealed that physicochemical and conformational properties of fusions improved while the structures possessed prolonged PAS sequences. Molecular docking results showed that the highest binding affinity to uric acid belonged to uricase-PAS1-100 by the formation of six hydrogens and four non-hydrogen bonds. Altogether, the results indicated that the PASylationprocess would be promising upon the production of urate oxidase with improved solubility and stability.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Abbas Najjari
Enzyme Technology Laboratory, Department of Biochemistry,Genetic and Metabolism Research Group, Pasteur Institute of Iran,Tehran, Iran.


| Hamzeh Rahimi
Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran.


| Seyed Ali Nojoumi
Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran.


| Eskandar Omidinia
Enzyme Technology Laboratory, Department of Biochemistry,Genetic and Metabolism Research Group, Pasteur Institute of Iran,Tehran, Iran.
نشانی اینترنتی http://ijmcmed.org/browse.php?a_code=A-10-2568-1&slc_lang=en&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Bioinformatic
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات