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International Journal of Molecular and Cellular Medicine، جلد ۱۰، شماره ۲، صفحات ۱۱۳-۱۲۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Mesenchymal Stem Cells cause Telomere Length Reduction of Molt-4 Cells via Caspase-3, BAD and P53 Apoptotic Pathway |
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| چکیده انگلیسی مقاله |
Mesenchymal stem cells (MSCs) as undifferentiated cells are specially considered in cell-based cancer therapy due to unique features such as multi-potency, pluripotency, and self-renewal. A multitude of cytokines secreted from MSCs are known to give such multifunctional attributes, but details of their role are yet to be unknown. In the present study, MSCs were cultured, characterized and co-cultured with Molt-4 cells as acute lymphoblastic leukemia cell line in a trans-well plate. Then, cultured Molt-4 alone and Molt-4 co-cultured with MSCs (10:1) were collected on day 7 and subjected to real time-PCR and Western blotting for gene and protein expression assessment, respectively. Ki-67/caspase-3 as well as telomere length were investigated by flow cytometry and real time-PCR, respectively. The results showed that MSCs caused significant decrease in telomere length as well as hTERT gene expression of Molt-4 cells. Also, gene and protein expression of BAD and P53 were significantly increased. Furthermore, the flow cytometry analysis indicated the decrease and increase of the Ki-67 and caspaspase-3 expression, respectively. It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis via caspase-3, BAD, and P53 expression. In addition, the decrease of telomere length is another effect of MSCs on Molt-4 leukemic cells. |
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| کلیدواژه‌های انگلیسی مقاله |
Mesenchymal stem cells, telomere length, hTERT, BAD, P53, caspase-3, apoptotic pathway |
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| نویسندگان مقاله |
| Hamid Reza Heidari
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Ezzatollah Fathi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
| Soheila Montazersaheb
Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Ayoub Mamandi
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
| Raheleh Farahzadi
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Soran Zalavi
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
| Hojjatollah Nozad Charoudeh
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-3464-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Stem Cell |
| نوع مقاله منتشر شده |
Original Article |
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