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International Journal of Molecular and Cellular Medicine، جلد ۱۰، شماره ۱، صفحات ۴۵-۵۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Knockdown of c-MYC Controls the Proliferation of Oral Squamous Cell Carcinoma Cells in vitro via Dynamic Regulation of Key Apoptotic Marker Genes |
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| چکیده انگلیسی مقاله |
Oral squamous cell carcinoma (OSCC) is the most common malignant epithelial cancer occurring in the oral cavity, where it accounts for nearly 90% of all oral cavity neoplasms. The c-MYC transcription factor plays an important role in the control of programmed cell death, normal-to-malignant cellular transformation, and progression of the cell cycle. However, the role of c-MYC in controlling the proliferation of OSCC cells is not well known. In this study, c-MYC gene was silenced in OSCC cells (ORL-136T), and molecular and cellular responses were screened. To identify the pathway through which cell death occurred, cytotoxicity, colony formation, western blotting, caspase-3, and RT-qPCR analyzes were performed. Results indicated that knockdown of c-MYC has resulted in a significant decrease in the cell viability and c-MYC protein synthesis. Furthermore, caspase-3 was shown to be upregulated leading to apoptosis via the intrinsic pathway. In response to c-MYC knockdown, eight cell proliferation-associated genes showed variable expression profiles: c-MYC (-21.2), p21 (-2.5), CCNA1(1.8), BCL2 (-1.4), p53(-3.7), BAX(1.1), and CYCS (19.3). p27 expression was dramatically decreased in c-MYC-silenced cells in comparison with control, and this might indicate that the relative absence of c-MYC triggered intrinsic apoptosis in OSCC cells via p27 and CYCS. |
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| کلیدواژههای انگلیسی مقاله |
Oral squamous cell carcinoma, siRNA, c-MYC, knockdown, p27, CYCS |
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| نویسندگان مقاله |
| Hussein Sabit
Department of Genetics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
| Huseyin Tombuloglu
Department of Genetics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
| Emre Cevik
Department of Genetics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
| Shaimaa Abdel-Ghany
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
| Engy El-Zawahri
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
| Amr El-Sawy
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
| Sevim Isik
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul, Turkey.
| Ebtesam Alsuhaimi
Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-3084-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cancer |
| نوع مقاله منتشر شده |
Original Article |
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