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International Journal of Molecular and Cellular Medicine، جلد ۹، شماره ۳، صفحات ۲۳۴-۲۴۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Differential Biological Behavior of Fibroblasts and Endothelial Cells under Aloe Vera Gel Culturing |
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| چکیده انگلیسی مقاله |
Aloe vera is used for its large variety of biological activities such as wound healing, anti-fungal, anti-inflammatory, hypoglycemic, immunomodulatory, gastroprotective, and anti-cancer. Although the beneficial effects of Aloe vera on wound healing have been proven, little is known about its effects at the cellular level. In this study, we evaluated the angiogenic and migrative effects of Aloe vera gel on fibroblasts and endothelial cells. Fibroblasts and endothelial cells were cultured in monolayer conditions with low glucose DMEM with 10% serum and 1% penicillin-streptomycin. Fresh and mature leaves of Aloe vera were used for gel preparation. Cell proliferation and morphology were studied by an inverted microscope. The migration of fibroblasts was assessed by scratch assay. MTT assay was performed for cell viability assessment, and real-time RT-PCR was used for evaluation of PECAM-1, integrin α1 and β1 transcription. After two days, the protein level of PECAM-1 was detected by flow cytometry. Our results showed that Aloe vera has a higher proliferative effect on fibroblasts in comparison with endothelial cells. Aloe vera also induced the migration of fibroblasts. The viability of both types of cells was similar to control ones. Integrin α1, β1 and PECAM-1 gene expression increased significantly (P < 0.005) in Aloe vera treated fibroblasts and endothelial cells in comparison with the control groups. However, the expression of these genes was significantly higher in fibroblasts in compareison with endothelial cells. Protein levels of PECAM-1 showed no change in both cell types upon Aloe vera treatment. Aloe vera gel induced angiogenic and cell adhesion properties in fibroblasts more than endothelial cells. Further investigations are needed to show the main role of fibroblasts rather than endothelial cells in wound healing by Aloe vera administration. |
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| کلیدواژه‌های انگلیسی مقاله |
Aloe vera gel, fibroblast, endothelial cells, integrin, PECAM-1 |
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| نویسندگان مقاله |
| Saba Shafaie
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
| Sina Andalib
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
| Hajar Shafaie
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz.
| Azadeh Montaseri
Department of Anatomical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
| Mahdi Tavakolizadeh
Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-2950-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cell Biology |
| نوع مقاله منتشر شده |
Original Article |
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