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International Journal of Molecular and Cellular Medicine، جلد ۱۱، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
miR-33-5p Regulates CREB to Induce Morphine State-dependent Memory in Rats: Interaction with µ Opioid Receptor |
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| چکیده انگلیسی مقاله |
The aim of the present study was to examine the hypothesis that miR-33-5p attenuates morphine state-dependent (StD) memory via the µ opioid receptor by regulating cyclic AMP response element-binding protein (CREB). The effects of post-training morphine and morphine StD memory and their interaction with pre-test naloxone were evaluated using a single-trial inhibitory avoidance paradigm. Then, the hippocampal miR-33-5p gene and pCREB/CREB protein expression profiles were evaluated using quantitative real-time PCR and western blotting, respectively. We found that while post-training morphine and morphine StD memory respectively up- and down-regulate the miR-33-5p expression profile in the hippocampus, the reverse results are true for the expression of pCREB/CREB. Pre-test naloxone antagonized the response. Overall, our findings suggest that the expression levels of miR-33-5p in the hippocampus set the basis for morphine StD memory with low miR-33-5p enabling state dependency. The mechanism is mediated via miR33-5p and CREB signaling with the interaction of the µ opioid receptor. This finding may be used as a potential strategy for ameliorating morphine-induced memory-related disorders. |
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| کلیدواژه‌های انگلیسی مقاله |
miR-33-5p, CREB, state-dependent memory, opioids, rats |
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| نویسندگان مقاله |
| Sadegh Moradi-Vastegani
Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan.
| Behrang Alani
Department of Applied Cell Sciences, School of Medicine, Kashan University of Medical Sciences, Kashan.
| Khatereh Kharazmi
Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan.
| Abolfazl Ardjmand
Institute for Basic Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan.
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-4948-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Neurosciences |
| نوع مقاله منتشر شده |
Original Article |
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