|
|
International Journal of Molecular and Cellular Medicine، جلد ۶، شماره ۱، صفحات ۰-۰
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
MeDIP Real-Time qPCR has the Potential for Noninvasive Prenatal Screening of Fetal Trisomy 21 |
|
| چکیده انگلیسی مقاله |
This study aimed to verify the reliability of the 7 tissue differentially methylated regions used in the methylated DNA immunoprecipitation (MeDIP) real-time quantitative polymerase chain reaction (real-time qPCR) based approach of fetal DNA in maternal blood to diagnosis of fetal trisomy 21. Forty pregnant women with high risk pregnancy who were referred after first or second trimester screening tests, were selected randomly. For each sample whole DNA extraction (mother and fetus), fragmentation of DNA, immunoprecipitation of methylated DNA and real- time qPCR using 7 primer pairs, was performed. D-value for each sample was calculated using the following formula D= -4.908+ 0.254 XEP1+ 0.409 XEP4+ 0.793 XEP5+ 0.324 XEP6+ 0.505 XEP7+ 0.508 XEP9+ 0.691 XEP12. In all normal cases, D value was negative, while it was positive in all trisomy cases. Therefore, all normal and trisomy 21 cases were classified correctly which correspond to 100% specificity and 100% sensitivity for this method. The MeDIP real-time qPCR method has provided the opportunity for noninvasive prenatal diagnosis of fetal trisomy 21 to be potentially employed into the routine practice of diagnostic laboratories. |
|
| کلیدواژه‌های انگلیسی مقاله |
|
|
| نویسندگان مقاله |
محمد کاظمی | mohammad kazemi
department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
منصور صالحی | mansoor salehi
department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
مجید خیرالهی | majid kheirollahi
pediatric inherited diseases research center, research institute for primordial prevention of noncommunicable disease, isfahan university of medical sciences, isfahan, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
|
|
| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-781-2&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/245/article-245-331291.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Genetics & Disease |
| نوع مقاله منتشر شده |
Original Article |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|