| چکیده انگلیسی مقاله |
Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1 – Sham/saline solution, G2 – CAE/control, G3 – acetic acid/control, G4 – curative-CAE treatment and G5 – preventive-CAE treatment. 8 days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) by immuno-histochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD. |
| نویسندگان مقاله |
maurício mercaldi pastrelo | maurício mercaldi pastrelo
department of pathology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
کارلا caroline dias ribeiro | carla caroline dias ribeiro
department of medicine, discipline of gastroenterology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
joselmo willamys duarte | joselmo willamys duarte
department of pathology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
andréa pitelli boiago gollucke | andréa pitelli boiago gollucke
department of biosciences, universidade federal de são paulo, santos, brazil.
ricardo artigiani neto | ricardo artigiani neto
department of pathology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
دانیال اراکی ribeiro | daniel araki ribeiro
department of biosciences, universidade federal de são paulo, santos, brazil.
مقاله jankiel miszputen | sender jankiel miszputen
department of medicine, discipline of gastroenterology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
celina tizuko fujiyama oshima | celina tizuko fujiyama oshima
department of pathology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
آنا paula ribeiro paiotti | ana paula ribeiro paiotti
department of pathology, universidade federal de são paulo, escola paulista de medicina, são paulo, brazil.
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