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International Journal of Molecular and Cellular Medicine، جلد ۴، شماره ۱، صفحات ۵۴-۵۹
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter? |
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| چکیده انگلیسی مقاله |
The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was accomplished in order to optimize the RNA extraction methods and compare the tissue amounts or tissues quality. Relatively, the cancerous tissues of human's stomach in fresh and frozen conditions and also the mouse's fresh tissue were studied. Some factors like the amount of samples, efficacy differences of diverse extraction's buffers (TriPure, Trizol) and also the efficacy of b-mercaptoethanol were compared and investigated. The results indicated that the less amount (1-2 mg) compared to other amounts (2-5 mg, 5-15 mg) yielded the best quality and the RNA's bands (5S, 18S, 28S) were observed perfectly. Relatively, comparing and measuring some kinds of buffers (Trizol, TriPure) indicated no difference in RNA extraction quality. The last investigated factor was the effect of b- mercaptoethanol which was used along with TriPure in order to remove the RNAse. Conclusively, no effective impression was observed. |
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| کلیدواژه‌های انگلیسی مقاله |
RNA extraction, frozen tissue, cancerous tissues |
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| نویسندگان مقاله |
علی اکبر صمدانی | ali akbar samadani
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
نوین نیک بخش | novin nikbakhsh
department of surgery, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
صادق فتاحی | sadegh fatahi
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
سید محسن آقاجانپور میر | seyed mohsen aghajanpour mir
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
سیده نرگس موسی کانی | seyedeh narges mosavi kani
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
رقیه پورباقر | rogheyeh pourbagher
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
زینب عابدیان | zeynab abediyan
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
هاله اخوان نیاکی | haleh akhavan niaki
cellular and molecular biology research center, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-371-1&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/245/article-245-331301.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Medical Pharmacology |
| نوع مقاله منتشر شده |
Original Article |
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