| چکیده انگلیسی مقاله |
Colorectal cancer (CRC), which is among the most prevalent cancers worldwide, is caused by environmental and genetic factors. It has been shown that the p53 gene is associated with CRC pathogenesis; moreover, Zyxin (ZYX) may play a role in p53 level and activity. Therefore, the present research aimed to investigate the levels of P53 and ZYX genes and proteins in CRC tumor samples. Cancerous tissues (n=31) and matched non-cancerous tissues (n=31) were randomly obtained from 31 patients with CRC. Total RNA was extracted using RNXplus, and gene expressions were assessed by Real-time PCR. Furthermore, the Western blot technique was used to investigate the expression of ZYX and P53 proteins. The obtained results revealed that the expression of ZYX and p53 genes in cancerous tissues showed no significant difference compared to matched non-cancerous tissues. On the other hand, measuring protein expression using the Western blotting technique indicated that the ZYX (P=0.0081) and p53 (P=0.0065) expression in tumor tissues significantly decreased compared to those in matched non-cancerous tissues. Correlation analysis indicated a significant correlation between ZYX and P53 proteins (r=0.746, P=0.013). Based on our findings, ZYX might have a suppressive function in CRC and is associated with P53. |
| نویسندگان مقاله |
| Hossein Mohammadi
Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Ebrahim Shakiba
Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Rezvan Rostampour
Clinical Research Development Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Kiana Bahremand
Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Mohammad Taghi Goodarzi
Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
| Homayoon Bashiri
Department of Internal Medicine, Infectious Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Khadijeh Najafi Ghobadi
Department of Biostatistics, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran.
| Soheila Asadi
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
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