| چکیده انگلیسی مقاله |
Previous researches revealed that Jianpi-Qinghua Formula (JPQH) exhibited anti-cancer activity. However, the pharmacological effect of JPQH on HBV-positive hepatocellular carcinoma (HCC) remains unknown. Cisplatin chemotherapy serves as a major treatment for HCC. Therefore, the aim of this study was to investigate whether JPQH or in combination with cisplatin therapy could inhibit the proliferation of HepG2.2.15 cells and elucidate the underlying mechanisms associated with the effect of cisplatin on HepG2.2.15 cells. We found that JPQH alleviated carbon tetrachloride (CCl4)-induced liver fibrosis and hepatitis in vivo. In vitro expriements, cisplatin effectively enhanced the suppression of HepG2.2.15 HCC cell proliferation and decreased their migration capacity. Moreover, JPQH could reduce CCl4-induced AST and ALT levels, as well as serum markers for liver fibrosis (including PⅢNP, C-Ⅳ, LN, HA levels) and hepatitis markers (including TBIL, IL6, and IL-1β levels). JPQH also induced cellular apoptosis by downregulating the expression levels of proteins such as Bcl-2 and IL-6. Additionally, JPQH could downregulate HBV expression. These findings collectively demonstrate that JPQH may inhibit the proliferation of HepG2.2.15 cells through suppression of HBV replication.
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| نویسندگان مقاله |
| Jianhua Xue
Department of Infectious Diseases, Hospital for Infectious Diseases of Pudong District, Shanghai, 201299, P.R. China.
| Beifen Zhong
Urologic Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, P.R. China.
| Lifeng Wang
Department of Infectious Diseases, Hospital for Infectious Diseases of Pudong District, Shanghai, 201299, P.R. China
| Xiaofeng Hu
Department of Infectious Diseases, Hospital for Infectious Diseases of Pudong District, Shanghai, 201299, P.R. China
| Yinhua Zhang
Department of Infectious Diseases, Hospital for Infectious Diseases of Pudong District, Shanghai, 201299, P.R. China
| Minfang Zhu
Pharmacy Department, Hospital for Infectious Diseases of Pudong District, Shanghai, 201299, P.R. China.
| Jianjie Chen
Department of infectious diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China.
| Hui Zhao
Shanghai Pudong New Area Mental Health Center,Shanghai,200124,P.R. China.
| xiangxiang wu
Department of Rheumatism and Immunology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, P.R. China.
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