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International Journal of Molecular and Cellular Medicine، جلد ۶، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from Enteroinvasive Escherichia coli (EIEC) Invasion |
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| چکیده انگلیسی مقاله |
Probiotic microorganisms have attracted a growing interest for prevention and therapy of gastrointestinal disorders. Many probiotic strains have been shown to inhibit growth and metabolic activity of enteropathogenic bacteria as well as their adhesion and invasion to intestinal cells. In the present study, we evaluated the interference of bacteria-free supernatants (BFS) of cultures belonging to sixteen strains of lactobacilli and bifidobacteria, with invasion of enteroinvasive Escherichia coli (EIEC) strain, by using human colonic adenocarcinoma cell lines, T84 and Caco2 cells. To assess invasion of Caco-2 and T84 cells by EIEC, and measure the number of pathogens inside the enterocytes, the gentamicin protection assay was conducted. In addition, three different invasion inhibition assays were designed; namely co-incubation, pre-incubation and treatment with the BFS of probiotics. Data obtained and theoretical calculation showed that the most effective assay in the prevention of pathogen invasion was treatment with BFS. Besides, co-incubation assay was more valid than pre-incubation assay in invasion prevention. The obtained results suggest that probiotics may produce some metabolites that strongly prevent invasion of enteroinvasive E.coli into the small and large intestine. Also, probiotics are able to compete with or exclude pathogen invasion. |
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| نویسندگان مقاله |
زهره خدایی | zohreh khodaii
dietary supplements and probiotic research center, alborz university of medical sciences, karaj, iran.
سید محمد حسین قادریان | sayyed mohammad hossein ghaderian
urogenital stem cell research center, shahid beheshti university of medical sciences, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
محبوبه مهربانی نطنزی | mahboobeh mehrabani natanzi
dietary supplements and probiotic research center, alborz university of medical sciences, karaj, iran.
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| نشانی اینترنتی |
http://ijmcmed.org/browse.php?a_code=A-10-1241-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Other |
| نوع مقاله منتشر شده |
Original Article |
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