Caspian Journal of Internal Medicine، جلد ۵، شماره ۲، صفحات ۱۰۹-۱۱۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Efficacy of multiplex PCR procedure for Iranian Streptococcus pneumoniae isolates
چکیده انگلیسی مقاله Background: Identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. The purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex PCR for the diagnosis of streptococcus pneumonia serotypes. Methods: All isolated S. pneumonia from suspected patients in Tehran and Isfahan Hospitals from June to December of 2012 were evaluated. All specimens and their serotypes were identified through a conventional method and specific antisera. Serotype specific multiplex PCR was applied and ran in seven reactions consisting of 34 S. pneumonia primer pairs plus a primer pair as an internal control for this purpose. Results: Overall, 14 genotype specific serotypes (including two subtypes for 19 and 23) were detected and had identical results with stereotyping method except for serotype 28 and one of the identified serotype 23. The serotypes 19, 6 and 23 were dominant with the frequency of 51.8%. A cross reactivity was also observed between genotypes 1 and 9A/9V. Conclusion: Applied multiplex PCR format can be suitable and cost effective tool for identification of S. pneumonia serotypes.
کلیدواژه‌های انگلیسی مقاله S. pneumoniae, Multiplex PCR, Identification
نویسندگان مقاله مسعود حاجیا | massoud hajia
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


محمد رهبر | mohamad rahbar
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


مرجان rahnami فرزامی | marjan rahnami farzami
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


علیرضا دولت یار | alireza dolatyar
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


محسن ایمانی | mohsen imani
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


رقیه saburian | roghieh saburian
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.


محمد فرزانه خواه | mohamad farzanehkhah
health reference laboratories research center, ministry of health amp;amp; medical education, tehran, iran.
نشانی اینترنتی http://www.caspjim.com/browse.php?a_code=A-10-62-178&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Infectious Diseases
نوع مقاله منتشر شده Short Communication
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